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通过磁性活化细胞分选分离的血管周围脂肪组织的脂肪细胞祖细胞的扩增和脂肪生成诱导

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

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通过磁性活化细胞分选分离的血管周围脂肪组织的脂肪细胞祖细胞的扩增和脂肪生成诱导

DOI:

10.3791/55818-v

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08:28 min

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June 30, 2017

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Kyan Thelen, Nadia Ayala-Lopez, Stephanie W. Watts, G. Andres Contreras

¹Department of Large Animal Clinical Sciences,Michigan State University, ²Department of Pharmacology and Toxicology,Michigan State University

Capitoli

00:05Titolo
00:50Adipocyte Progenitors Isolation
02:52Magnetic Activated Cell Sorting
05:13Cell Culture and Adipogenesis Induction
06:35Results: Representative Adipocyte Progenitor Cells (APCs) Differentiation Analysis
07:57Conclusion

Summary

Traduzione automatica

在这里，我们报告了使用磁激活细胞分选（MCS）从血管周围脂肪组织（PVAT）分离脂肪细胞祖细胞（APC）种群的方法。当与荧光活化细胞分选（FACS）相比时，该方法允许APC每克脂肪组织的APC分离增加。

Tags

Keywords: Adipocyte Progenitor Cells Magnetic Activated Cell Sorting Adipogenesis Stromal Vascular Fraction Collagenase Digestion Cell Filtration Erythrocyte Lysis

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