Rivista
/
Biologia
/
监测ER / SR钙释放与目标Ca<sup> 2+</sup>传感器捕捉器<sup> +</sup
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+
Please note that all translations are automatically generated. Click here for the English version.

监测ER / SR钙释放与目标Ca<sup> 2+</sup>传感器捕捉器<sup> +</sup

DOI:
10.3791/55822-v

•

12:30 min

•

May 19, 2017

•

, , ,
1Department of Chemistry, Center of Diagnostics and Therapeutics (CDT),Georgia State University

Capitoli

  • 00:05Titolo
  • 01:30Transfection of HEK293 Cells
  • 02:43Transfection of C2C12 Myoblast Cells
  • 04:10Preparation of Slide and Fluorescence Microscope
  • 06:54Imaging Drug-induced Ca2+ Transients and In Situ Kd Calibration
  • 10:14Results: In Situ Kd of CatchER+ in C2C12 Myoblast Cells
  • 11:32Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们提出了使用我们的靶向基因编码钙指示剂（GECI）CatchER +的方案 ，用于使用实时荧光显微镜监测HEK293和骨骼肌C2C12细胞的内质网/肌浆网（ER / SR）中的快速钙瞬变。还讨论了原位测量和校准的协议。

Tags

ER/SR Calcium ReleaseCatchER+HEK293 CellsC2C12 CellsCalcium SignalingCalcium TransientsGECICell TransfectionFluorescence ImagingCalcium-related Diseases

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/55822?article_type=v

拆装时间推移显微镜监测肌动蛋白
check_url/it/55822?article_type=v

载入果蝇与神经末梢钙指标
check_url/it/55822?article_type=v

加固，Eyetracking，生理监测的功能成像
check_url/it/55822?article_type=v

多巴胺的释放突触前货箱码头在个别的可视化与FFNs
check_url/it/55822?article_type=v

在人T淋巴细胞的全细胞钙释放激活的钙记录（CRAC）的电流
check_url/it/55822?article_type=v

使用一种基因编码的钙传感器来监测细胞凋亡过程中线粒体钙水平的动态变化
check_url/it/55822?article_type=v

活细胞钙成像相结合的siRNA介导的基因沉默标识CA 2 +泄漏通道内质网膜和监管机制
check_url/it/55822?article_type=v

致命的甲壳类动物长期毒性试验卤虫franciscana</em
check_url/it/55822?article_type=v

直接成像诱导ER钙有针对性的酯酶染料装载（TED）
check_url/it/55822?article_type=v

监测内质网钙稳态使用<em> Gaussia</em>荧光素酶SERCaMP

Read Article