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A Unified Methodological Framework for Vestibular Schwannoma Research
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JoVE Journal Ricerca sul cancro
A Unified Methodological Framework for Vestibular Schwannoma Research

A Unified Methodological Framework for Vestibular Schwannoma Research

DOI:
10.3791/55827-v

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08:43 min

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June 20, 2017

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, , , , ,
1Eaton Peabody Laboratories, Department of Otolaryngology,Massachusetts Eye and Ear, 2Department of Otolaryngology,Harvard Medical School, 3Department of Otolaryngology, Vienna General Hospital,Medical University of Vienna, 4Program in Speech and Hearing Bioscience and Technology,Harvard Medical School

Capitoli

  • 00:05Titolo
  • 00:57Receipt and Cleasing of a Vestibular Schwannoma or Great Auricular Nerve Sample
  • 02:25Collection of Vestibular Schwannoma or Great Auricular Nerve Secretions
  • 03:52Primary Human Vestibular Schwannoma Culture
  • 07:07Results: Representative Images of Primary Human Vestibular Schwannoma Cultures
  • 07:48Conclusion

Summary

Traduzione automatica

Traduzione automatica

The goal of this protocol is to outline the collection and processing of human surgical samples for multiple downstream applications in vestibular schwannoma and Schwann cell research.

Tags

Keywords: Vestibular SchwannomaPrimary Cell CultureTumor SecretionsBiomolecular AssaysMinimally Manipulative CultureTherapeutic CandidatesRNA PreservationProtein ExtractionFixationDMEM F12 MediumTumor Secretion Collection

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