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An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers
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JoVE Journal Medicina
An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers

DOI:
10.3791/55833-v

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06:34 min

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December 01, 2017

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, , , , , ,
1Applied Microbiology Research, Department of Biomedicine,University of Basel, 2Department of Biosystems Science and Engineering,ETH Zurich, 3Swiss Institute of Bioinformatics, 4Clinical Microbiology,University Hospital Basel

Capitoli

  • 00:05Titolo
  • 00:54Hemagglutination Inhibition (HI) Assay
  • 03:28Results: Representative Vacination-induced Antibody Responses Against Influenza
  • 05:11Conclusion

Summary

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The presented protocols describe how to perform a hemagglutination inhibition assay to quantify influenza-specific antibody titers from serum samples of influenza vaccine recipients. The first assay determines optimal viral antigen concentrations by hemagglutination. The second assay quantifies influenza-specific antibody titers by hemagglutination inhibition.

Tags

Keywords: Hemagglutination Inhibition AssayInfluenza-specific Antibody TitersVaccine-mediated ImmunityNeutralizing AntibodiesMouse SerumCulture SupernatantsAntigen SolutionRDE Treated Serum SamplesAntiserumSerial DilutionsRed Blood Cell SolutionIncubation TimeHemagglutination

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