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Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain
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Biochimica
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JoVE Journal Biochimica
Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain

Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain

DOI:
10.3791/55835-v

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09:35 min

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October 24, 2017

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, ,
1Department of Biochemistry,Emory School of Medicine

Capitoli

  • 00:05Titolo
  • 00:42Homogenization and Fractionation
  • 04:54Western Blotting
  • 07:48Results: Detection of AD-related Detergent-insoluble Protein Aggregates from the Human Postmortem Brain
  • 09:05Conclusion

Summary

Traduzione automatica

Traduzione automatica

An abbreviated fractionation protocol for the enrichment of detergent-insoluble protein aggregates from human postmortem brain is described.

Tags

Keywords: Detergent-insoluble Protein AggregatesHuman Postmortem BrainNeurodegenerationMisfolded Protein AggregatesGray MatterLow-salt BufferSodium ChlorideSarkosylSonicationProtein Concentration

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