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A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
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Medicina
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JoVE Journal Medicina
A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells

A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells

DOI:
10.3791/55841-v

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09:05 min

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August 07, 2017

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, , , , ,
1Department of Internal Medicine,Leiden University Medical Centre

Capitoli

  • 00:05Titolo
  • 00:53Kidney Isolation
  • 03:48Kidney Perivascular Stromal Cell (kPSC) Culture
  • 05:46Functional Assessment
  • 06:57Results: Representative Human kPSC Characterization
  • 07:49Conclusion

Summary

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Here we present a novel clinical grade isolation and culture method for kidney Perivascular Stromal Cells (kPSCs) based on whole organ perfusion with digestive enzymes and NG2-cell enrichment. With this method, it is possible to acquire sufficient cell numbers for cellular therapy.

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Keywords: Kidney Perivascular Stromal CellsKPSCClinical Grade Isolation MethodRegenerative MedicineCell TherapyPerfusion TrayCollagenaseDNAseCell SuspensionKidney Cell Isolation

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