/

/

从孤立的大鼠心脏的间质渗出物收集的倒置心脏模型

JoVE Journal
Medicina

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Medicina

The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart

Please note that all translations are automatically generated. Click here for the English version.

从孤立的大鼠心脏的间质渗出物收集的倒置心脏模型

DOI:

10.3791/55849-v

•

07:59 min

•

June 20, 2017

•

Kezhe Tan*^1,2, Zhaoping Ding*^1,2, Bodo Steckel, Sonja Hartwig, Stefan Lehr, Xiaoming Deng, Jürgen Schrader

¹Department of Anesthesiology, Changhai Hospital,Second Military Medical University, ²Department of Molecular Cardiology,Heinrich Heine University, ³Department of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center,Heinrich Heine University

Capitoli

00:05Titolo
01:08Preparation of the Latex Cap and Intraventricular Balloon
03:06Isolation and Cannulation of the Heart
04:34Reversed-heart Model
06:24Results: Protein and Purine Analysis of Cardiac Transudate
07:33Conclusion

Summary

Traduzione automatica

该方案描述了从分离的灌注大鼠心脏中收集心脏间质液的方法。为了将间质渗出物与冠状静脉流出物灌注液物理分离，Langendorff灌注的心脏被倒置，并且使用软胶乳帽收集在心脏表面上形成的渗出物（间质液体）。

Tags

Inverted Heart Model Interstitial Transudate Collection Isolated Rat Heart Paracrine Factors Interstitial Fluid Space Cardiac Cells Inflammation Regeneration Infarcted Myocardium Latex Heart Cover Mold Liquid Latex Silicon Tubing Cardiac Transudate Ventricular Balloon Pressure Transducer Langendorff Perfusion System

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

主动脉原位移植大鼠模型，研究慢性血管病变的发展

心肌缺血再灌注损伤的通过左前降支结扎小鼠模型

的全人工心脏的先天性心脏病植入

一个孤立的工作心脏系统的大型动物模型

的隔离方法<em>离体</em>肺灌注的大鼠模型：教训制定鼠EVLP课程中学

心肌顿抑相关的小鼠离体心脏模型心脏停搏

无麻醉大鼠尾静脉血采集系统的研究

研究小鼠心肌梗死的低温损伤模型

大鼠中三极重化控制体积过载的图像引导透射斜线阀传单穿刺模型

腹部小鼠心脏移植模型中的血液回路重建

Read Article