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Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

DOI:
10.3791/55857-v

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07:35 min

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June 07, 2017

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, , , ,
1Department of Microbiology, Immunology, and Pathology,Colorado State University, 2Division of Vector-Borne Diseases,Centers for Disease Control and Prevention

Capitoli

  • 00:05Titolo
  • 00:40Preparation of In Vitro Transcribed RNA
  • 02:54Rescue of Infectious Virus by Electroporation
  • 05:56Results: In Vitro and In Vivo Characteristics of Clone-derived Virus
  • 07:02Conclusion

Summary

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This protocol describes the recovery of infectious Zika virus from a two-plasmid infectious cDNA clone.

Tags

Keywords: Zika VirusInfectious CloneRecombinant VirusFlavivirusPlasmid DigestionLigationIn Vitro TranscriptionVero CellsElectroporation

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