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Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
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Biochimica
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JoVE Journal Biochimica
Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

DOI:
10.3791/55858-v

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10:01 min

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December 04, 2017

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, ,
1Department of Chemistry,University of Oxford, Inorganic Chemistry Laboratory

Capitoli

  • 00:05Titolo
  • 00:50Immobilization of E. coli Hydrogenase 1 on Carbon Black Particles
  • 03:02Protein Film Infrared Electrochemistry (PFIRE) Setup
  • 06:07Activation of E. coli Hydrogenase 1 and PFIRE Measurements
  • 07:53Results: PFIRE Measurements of E. coli Hydrogenase 1
  • 09:22Conclusion

Summary

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Here, we describe a technique, protein film infrared electrochemistry, which allows immobilized redox proteins to be studied spectroscopically under direct electrochemical control at a carbon electrode. Infrared spectra of a single protein sample can be recorded at a range of applied potentials and under a variety of solution conditions.

Tags

Protein Film Infrared Electrochemistry[NiFe] HydrogenaseH2 OxidationRedox EnzymeBiophysicsBioelectrochemistryCarbon BlackBuffer ExchangeProtein AbsorptionPFIRE Measurements

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