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Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons
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Neuroscienze
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JoVE Journal Neuroscienze
Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

DOI:
10.3791/55871-v

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10:36 min

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November 06, 2017

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, , ,
1Lady Davis Institute for Medical Research,Jewish General Hospital, 2Integrated Program in Neuroscience,McGill University, 3Department of Cellular and Molecular Medicine,University of Ottawa, 4Department of Oncology, Faculty of Medicine,McGill University

Capitoli

  • 00:05Titolo
  • 01:11Brain Dissection and Isolation of the Cerebellum
  • 03:11Mouse Cerebellar Granule Neuron Isolation and Culturing
  • 06:38Modeling Neuronal Injury
  • 08:09Results: Representative Images of Cerebellar Granule Neurons Infected with Different MOIs
  • 09:36Conclusion

Summary

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This protocol describes a simple method for isolating and culturing primary mouse cerebral granule neurons (CGNs) from 6-7 day old pups, efficient transduction of CGNs for loss and gain of function studies, and modelling NMDA-induced neuronal excitotoxicity, low-potassium-induced cell death, DNA-damage, and oxidative stress using the same culture model.

Tags

Cerebellar Granule NeuronsNeuronal DeathNeurodegenerationPrimary Cell CultureExcitotoxicityOxidative StressDNA DamageDevelopmental EventsNeuronal ActivityMorphogenesisGrowth FactorsMouse Brain DissectionCerebellum IsolationMeningeal Removal

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