/
/
Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 00:46Collection and Concentration of Viruses Expressing Metastatic Factor
- 02:26Viral Titer Determination
- 04:37Intracardiac Injection
- 07:24Results: Detection of Metastatic Pancreatic β-cells by Immunostaining
- 08:16Conclusion
We present a protocol to demonstrate a novel somatic gene transfer system utilizing RIP-Tag; RIP-tva mouse model to study the function of genes in metastasis. The avian retroviruses are delivered intracardiacally to ensure gene transfer into pre-malignant, noninvasive lesions of pancreatic β cells in adult mice.
