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定量荧光测量全球本地化翻译
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Biochimica
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JoVE Journal Biochimica
Quantitative Immunofluorescence to Measure Global Localized Translation
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定量荧光测量全球本地化翻译

DOI:
10.3791/55909-v

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09:13 min

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August 22, 2017

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1Centre de Recherche sur le Cancer de l’Université Laval, Faculté de Médecine, Département de Biologie moléculaire, biochimie médicale et pathologie,Université Laval, 2CRCHU de Québec: L’Hôtel-Dieu de Québec

Capitoli

  • 00:05Titolo
  • 00:45Cell Immunostaining
  • 03:34Image Acquisition
  • 04:49Signal Quantification
  • 06:50Results: Proper Puromycin Treatment and Analysis in MRC-5 and Other Cell Types
  • 08:16Conclusion

Summary

Traduzione automatica

Traduzione automatica

这份手稿描述了一种方法来可视化和量化本地化的翻译事件亚细胞的车厢内。提出这份手稿的做法需要基本的共焦成像系统和试剂，并且是快速和具有成本效益。

Tags

Quantitative ImmunofluorescenceLocalized TranslationCell AdhesionPuromycinAnti-puromycin AntibodyFluorophore-conjugated Secondary AntibodiesCell MigrationCell AdhesionEarly Drug ResponseMRC-5 CellsCycloheximideFormaldehyde FixationTriton X-100 PermeabilizationBSA BlockingTween-20 Washing

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