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Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall
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Biochimica
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JoVE Journal Biochimica
Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall

Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall

DOI:
10.3791/55910-v

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07:51 min

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June 10, 2017

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, ,
1Beijing Key Laboratory of Lignocellulosic Chemistry,Beijing Forestry University

Capitoli

  • 00:05Titolo
  • 00:44Sample Preparation
  • 03:37Spectral Acquisition
  • 05:53Results: Raman Imaging Data Analysis
  • 06:49Conclusion

Summary

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This protocol aims to present a general method to visualize lignin, cellulose, and hemicellulose in plant cell walls using Raman imaging and multivariate analysis.

Tags

Raman ImagingMultivariate AnalysisPlant Cell WallLigninCelluloseHemicellulosePlant BiomassPEG InfiltrationMicrotome SectioningLignin RemovalSample Preparation

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