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通过RNA定位蝗虫天线中的气味受体基因<em>原位</em>杂交
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
DOI:

09:30 min

July 13, 2017

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Capitoli

  • 00:05Titolo
  • 00:55Preparation of Cryostat Sections
  • 01:59Fixing Sections and Hybridization
  • 03:30Washing, Blocking, and Immunohistochemistry
  • 05:10Staining
  • 07:02Results: Neuronal Identity of Antennal Cells Expressing LmigORs by Chromogenic and TSA Detections
  • 08:27Conclusion

Summary

Traduzione automatica

本文描述了一种详细和高效的RNA 原位杂交方案,特别是对于低水平表达的气味受体(OR)基因以及其他基因,在使用地高辛(DIG)标记或生物素标记的探针的昆虫天线中。

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