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Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
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JoVE Journal Biochimica
Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

DOI:
10.3791/55932-v

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12:55 min

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October 10, 2017

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, ,
1Systems Biotechnology Research Center,KIST Gangneung Institute of Natural Products, 2Division of Bio-Medical Science & Technology, KIST School,Korea University of Science and Technology

Capitoli

  • 00:05Titolo
  • 01:01Cell Culture, Treatment, and Examination of Protein Expression
  • 02:53Phosphate Assay
  • 05:22Microtubule-binding Assay
  • 07:55Localization and Expression of Tau After Treatment of Cells with Curcumin
  • 10:23Results: Phosphorylation, Microtubule Binding, and Localization of Tau Protein in Colorectal Cancer Cells
  • 12:03Conclusion

Summary

Traduzione automatica

Traduzione automatica

This manuscript describes standard protocols for measuring tau hyperphosphorylation, measuring tau binding to microtubules, and localization of intracellular tau following drug treatments. These protocols can be used repetitively for screening drugs or other compounds that target tau hyperphosphorylation or microtubule binding.

Tags

Tau PhosphorylationMicrotubule BindingTau LocalizationConfocal MicroscopyColorectal Cancer CellsPhosphatase AssayMicrotubule-binding AssayTau ProteinHCT 116 CellsCurcuminLithium ChlorideRIPA BufferBradford AssayAlkaline Phosphatase

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