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大肠癌细胞中磷酸化和微管结合与 Tau 蛋白定位的测定

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Biochimica

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JoVE Journal Biochimica

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

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大肠癌细胞中磷酸化和微管结合与 Tau 蛋白定位的测定

DOI:

10.3791/55932-v

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12:55 min

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October 10, 2017

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Md Nazmul Huda², Erdenedolgor Erdene-Ochir², Cheol-Ho Pan²

¹Systems Biotechnology Research Center,KIST Gangneung Institute of Natural Products, ²Division of Bio-Medical Science & Technology, KIST School,Korea University of Science and Technology

Capitoli

00:05Titolo
01:01Cell Culture, Treatment, and Examination of Protein Expression
02:53Phosphate Assay
05:22Microtubule-binding Assay
07:55Localization and Expression of Tau After Treatment of Cells with Curcumin
10:23Results: Phosphorylation, Microtubule Binding, and Localization of Tau Protein in Colorectal Cancer Cells
12:03Conclusion

Summary

Traduzione automatica

本手稿描述了测量 tau tau, 测量 tau 结合到微管的标准协议, 以及药物治疗后细胞内 tau 的定位。这些协议可以重复使用, 以筛选药物或其他化合物的目标 tau 或微管结合。

Tags

Keywords: Tau Phosphorylation Microtubule Binding Tau Localization Confocal Microscopy Colorectal Cancer Cells Phosphatase Assay Microtubule-binding Assay Tau Protein HCT 116 Cells Curcumin Lithium Chloride RIPA Buffer Bradford Assay Alkaline Phosphatase

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