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小容积回收-灌注-浸没式室系统中维持的急性海马切片的突触可塑性记录

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Neuroscienze

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JoVE Journal Neuroscienze

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

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小容积回收-灌注-浸没式室系统中维持的急性海马切片的突触可塑性记录

DOI:

10.3791/55936-v

•

09:51 min

•

January 01, 2018

•

Weiguang Weng, Dongxue Li, Cheng Peng, Thomas Behnisch

¹The Institutes of Brain Science, the State Key Laboratory of Medical Neurobiology, the Collaborative Innovation Center for Brain Science,Fudan University

Capitoli

00:05Titolo
01:05Preparation of Acute Hippocampal Slices
02:21Modifications of the Carbogenation for the aCSF Recycling of Small Reservoirs
03:08Recording Synaptic Responses in a Submersion Slice Chamber
07:28Results: Representative Recordings of Activity-dependent Potentiaation of Synaptic Transmission Using Outflow-carbogenation and Reservoir-carbogenation
08:08Conclusion

Summary

Traduzione automatica

本协议描述了在少量回收的缓冲液中的氧水平的稳定, 以及在淹没的急性海马切片中记录活动依赖性突触可塑性的方法学方面。

Tags

Synaptic Plasticity Acute Hippocampal Slices Recycling-perfusion-submersion Chamber Carbogenation Field Potential Recordings Neuroplasticity Synaptic Transmission Memory Formation Oxygen Stabilization Acute Slice Preparation Vibratome Hippocampal Formation Recycling System Carbogen Supply

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