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一个<em秀丽隐杆线虫</em基于营养状态的铜反转测定
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JoVE Journal Neuroscienze
A Caenorhabditis elegans Nutritional-status Based Copper Aversion Assay
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一个<em秀丽隐杆线虫</em基于营养状态的铜反转测定

DOI:
10.3791/55939-v

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06:45 min

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July 26, 2017

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, ,
1Department of Biology,Queen’s University, 2Centre for Neuroscience,Queen’s University

Capitoli

  • 00:05Titolo
  • 00:42Preparation of Experimental Organisms
  • 01:15Preparation of Assay Plates
  • 02:31Chemotaxis Assay
  • 05:31Results: npr-9 Overexpression Nematodes Do Not Initiate Aversion to Copper
  • 06:21Conclusion

Summary

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在这里，我们提出了一种秀丽隐杆线虫特异性测定法，用于评估铜厌恶行为的变化和定位常见食物来源的能力，因为生物体从充足的营养状态进入饥饿的营养状态。

Tags

Caenorhabditis ElegansCopper Aversion AssayNutritional StatusBehavioral NeuroscienceL4 Stage NematodesOP50 E. ColiCopper(II)sulfateFood PatchMidline Barrier

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