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用于植物/微生物分子相互作用和信号的同时系统分析的水培共培养系统

JoVE Journal
Biologia

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JoVE Journal Biologia

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

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用于植物/微生物分子相互作用和信号的同时系统分析的水培共培养系统

DOI:

10.3791/55955-v

•

11:16 min

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July 22, 2017

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Naeem Nathoo², Mark A. Bernards, Jacqueline MacDonald, Ze-Chun Yuan³

¹London Research and Development Centre,Agriculture & Agri-Food Canada, ²Department of Biology,University of Western Ontario, ³Department of Microbiology and Immunology,University of Western Ontario

Capitoli

00:05Titolo
01:24Plant Seed Surface Sterilization
03:03Seed Germination and Semi-solid Cultivation of Plants
04:51Hydroponic Co-cultivation System
07:39Fluorescence Microscopy
08:24Secretome Profiling
09:10Results: Co-cltivation System Mimics Natural Environment
10:28Conclusion

Summary

Traduzione automatica

所描述的水培共培养系统支持具有金属筛网的完整植物，并将其与细菌共培养。然后可以单独收集植物组织，细菌和分泌的分子用于下游分析，同时允许研究植物宿主和相互作用的微生物或微生物群的分子响应。

Tags

Hydroponic Co-cultivation Plant-microbe Interactions Molecular Signaling Agrobacterium Tumefaciens Arabidopsis Thaliana Seed Sterilization Murashige And Skoog (MS) Medium Phyto Agar Plant-microbe Communication Reciprocal Signaling Abiotic Factors Natural Environment Simulation Single/multiple Microbial Species Cell Signaling Homeostasis

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