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3D Microtissues 用于可注射再生疗法和高通量药物筛选

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Bioingegneria

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JoVE Journal Bioingegneria

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

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3D Microtissues 用于可注射再生疗法和高通量药物筛选

DOI:

10.3791/55982-v

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11:28 min

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October 04, 2017

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Yaqian Li*^1,2, Xiaojun Yan*¹, Wei Liu*¹, Lyu Zhou³, Zhifeng You, Yanan Du²

¹Department of Biomedical Engineering, School of Medicine,Tsinghua University, ²Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, ³School of Life Sciences,Tsinghua University

Capitoli

00:05Titolo
01:18Fabrication of 3D Microcryogels
03:11Injectable 3D Microtissues for Treatment of Critical Limb Ischemia
07:09Assembly of Microtissue Array Chip for High-throughput Drug Screening
09:05Results: Microcryogels Used as a Therapeutic and for High-throughput Drug-screening
10:31Conclusion

Summary

Traduzione automatica

本协议描述了通过将微细加工与 cryogelation 技术相结合的方法制备弹性3D 大孔 microcryogels。在加载与细胞, 3D microtissues 生成, 这可以很容易地注入在体内, 以促进再生治疗或组装成阵列的体外高通量药物筛选。

Tags

Regenerative Therapy Drug Screening Cryogelation Gelatin Microcryogels PMMA Microstencil PDMS Ejector Pin Lyophilization High-throughput

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