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Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins
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JoVE Journal Neuroscienze
Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins

Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins

DOI:
10.3791/56016-v

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09:07 min

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August 15, 2017

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, ,
1Department of Molecular and Integrative Physiology,University of Illinois at Urbana-Champaign, 2Neuroscience Program,University of Illinois at Urbana-Champaign

Capitoli

  • 00:05Titolo
  • 01:08Preparation of an ECS Machine
  • 01:47Induction of Acute ECS
  • 03:07Homogenization and Fractionation of Rat Hippocampi
  • 05:20Isolation of the PSD from the Crude Membrane Protein Fraction
  • 06:32Results: Examination of Synaptic Proteins in Hippocampal S2, P2, and PSD Fractions from Rats that Received NS or Acute ECS
  • 07:55Conclusion

Summary

Traduzione automatica

Traduzione automatica

Electroconvulsive seizure (ECS) is an experimental animal model of electroconvulsive therapy for severe depression. ECS globally stimulates activity in the hippocampus, leading to synaptogenesis and synaptic plasticity. Here, we describe methods for ECS induction in rats and for subcellular fractionation of their hippocampi to examine seizure-induced changes in synaptic proteins.

Tags

Keywords: Electroconvulsive SeizureHippocampusPostsynaptic Density ProteinsNeuroplasticityPulse GeneratorSeizure InductionRacine ScaleHippocampal Fractionation

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