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Genetics

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JoVE Journal Genetics
High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster
 

High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster

Article DOI: 10.3791/56029-v • 08:28 min • September 13th, 2017
September 13th, 2017 •
1, 1, 1
1Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute

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This is a high fat diet feeding protocol to induce obesity in Drosophila, a model for understanding fundamental molecular mechanisms implicated in lipotoxicity. It also provides a high throughput triacylglyceride assay for measuring fat accumulation in Drosophila and potentially other (insect) models under various dietary, environmental, genetic or physiological conditions.

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Keywords: High Fat Diet High Throughput Triacylglyceride Assay Drosophila Melanogaster Obesity Metabolism Lipotoxicity Diabetes Metabolic Syndrome Liver Steatosis Cardiac Steatosis Drug Discovery High Fat Food Coconut Oil Food Preparation Fly Food Anesthesia Aging High Fat Diet Feeding Normal Food
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