Rivista
/
Biologia
/
Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.

Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.

DOI:
10.3791/56030-v

•

08:04 min

•

July 14, 2017

•

, , , , , ,
1Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology,Nanjing Agricultural University

Capitoli

  • 00:05Titolo
  • 00:55Isolation of Sialic Acids from Milk and from Liver Tissue
  • 03:26Preparation of a Mixed Sialic Acid Standard
  • 04:26Fluorescence Derivatization of Sialic Acids
  • 05:18HPLC Analysis of Sialic Acid Derivatives
  • 06:15Results: Quantification of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice
  • 07:05Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe a HPLC-based method for the determination of N-acetylneuraminic acid and N-glycolylenuraminic acid in mouse liver and milk.

Tags

Sialic AcidN-Acetylneuraminic AcidN-Glycolylneuraminic AcidCMAHLiverMilkHPLCGlycoscienceMouse ModelSample PreparationAnion Exchange Chromatography

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Quantitation of γH2AX Foci in Tissue Samples

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1

Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer

In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks

Milk Collection Methods for Mice and Reeves’ Muntjac Deer

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Read Article