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氮气气蚀和差速离心允许监测外周血膜蛋白在细胞中的分布
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Biochimica
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JoVE Journal Biochimica
Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells
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氮气气蚀和差速离心允许监测外周血膜蛋白在细胞中的分布

DOI:
10.3791/56037-v

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08:24 min

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August 18, 2017

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,
1Langone Medical Center,New York University

Capitoli

  • 00:05Titolo
  • 00:41Cell Harvesting
  • 01:35Nitrogen Cavitation
  • 03:31Separation of Cytosolic and Membrane Fractions
  • 05:22Results: Partitioning of Cellular Proteins in Membrane and Cytosolic Fractions
  • 07:07Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里我们目前用于洗涤剂免费培养的哺乳动物细胞基于氮空和随后的分离，胞浆和膜结合蛋白的超速离心同质化的协议。此方法是理想的监测分区之间可溶性的外周膜蛋白和膜组分。

Tags

Nitrogen CavitationDifferential CentrifugationPeripheral Membrane ProteinsCultured CellsSubcellular CompartmentalizationHEK293 CellsHomogenization BufferPressurizationProtease InhibitorCell DisruptionFractionationSoluble FractionMembrane Fraction

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