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小鼠第二腭融合的实时成像

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Live Imaging of Mouse Secondary Palate Fusion

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小鼠第二腭融合的实时成像

DOI:

10.3791/56041-v

•

06:10 min

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July 27, 2017

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Seungil Kim, Jan Prochazka, Jeffrey O. Bush

¹Department of Cell and Tissue Biology, Program in Craniofacial Biology and Institute for Human Genetics,University of California San Francisco, ²Laboratory of Transgenic Models Diseases,Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences

Capitoli

00:05Titolo
00:42Palate Explant Culture and Confocal Time-lapse Imaging
03:28Results: Representative Live Palate Explant Culture Imaging
05:16Conclusion

Summary

Traduzione automatica

在这里，我们提出了使用共聚焦显微镜的小鼠二次腭融合的活体成像方案。该方案可以与各种荧光报告小鼠系列结合使用，并可与途径抑制剂一起用于机械性的洞察。该协议可适用于其他开发系统中的实时成像。

Tags

Live Imaging Mouse Secondary Palate Fusion Confocal Microscopy Cranial Facial Development Palatal Shelves Midline Epithelia Seam Epithelial Convergence Explant Culture

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