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细菌显示文库的半自动化筛选肽亲和试剂的发现与分离结果分析
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
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细菌显示文库的半自动化筛选肽亲和试剂的发现与分离结果分析

DOI:
10.3791/56061-v

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13:49 min

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December 06, 2017

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, ,
1Sensors and Electron Devices Directorate,US Army Research Laboratory

Capitoli

  • 00:05Titolo
  • 01:00Negative vs Positive Sorting
  • 01:51AutoMACS Bacterial Display Library Biopanning Positive Sorting
  • 06:27FACS Analysis of Sorting Rounds
  • 11:04Results: Representative Peptide Affinity and Specificity Date, and Alignment and Consensus Determination for Sequencing Results
  • 12:46Conclusion

Summary

Traduzione automatica

Traduzione automatica

筛选细菌显示库是一种被证实的技术, 用于发现肽亲和试剂, 一种强有力的替代抗体。本文的半自动排序方法简化了筛选, 减少了误报的发生。在这里, 我们说明的思想过程和技术应用于评价候选人和最小化下游分析。

Tags

Keywords: Semi-automated BiopanningBacterial Display LibrariesPeptide Affinity ReagentsProtein TargetNegative SortingPositive SortingL-arabinose InductionDownstream Sequence Analysis

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