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生活成像的脑皮层细胞使用二维培养系统
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Neuroscienze
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JoVE Journal Neuroscienze
Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System
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生活成像的脑皮层细胞使用二维培养系统

DOI:
10.3791/56063-v

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10:12 min

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August 09, 2017

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, , , ,
1Brain Institute,Federal University of Rio Grande do Norte, 2Department of Biosystems Science and Engineering,ETH Zurich, 3The Solomon H. Snyder Department of Neuroscience,Johns Hopkins University

Capitoli

  • 00:05Titolo
  • 00:57Dorsolateral Telencephalon Microdissection and Cell Plating
  • 04:23Time-lapse Video Microscopy
  • 05:24Cell Tracking
  • 08:12Results: Cell Lineage of Single Progenitor Cells
  • 09:41Conclusion

Summary

Traduzione automatica

Traduzione automatica

生动的图像是研究在实时的细胞行为的有力工具。在这里，我们描述延时视频-显微镜的脑皮层细胞，允许颁布在沿袭前进过程中主要的神经干细胞分化的神经元和神经胶质的阶段详细的审查的协议。

Tags

Keywords: Live ImagingPrimary Cerebral Cortex Cells2D Culture SystemNeural Stem CellsNeuronsGlial CellsNeurodevelopmentCell LineageCell DivisionCell CycleCell GrowthEmbryonic BrainDorsolateral TelencephalonCell DissociationTrypsin-EDTAProliferation MediumPoly-D-lysine

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