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Counting Proteins in Single Cells with Addressable Droplet Microarrays
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Capitoli
- 00:05Titolo
- 00:55Prepare Apparatus for Dispensing Addressable Droplets
- 03:26Form Addressable Droplets and Load with Single Cells
- 07:42Data Analysis
- 09:39Results: Single Cell Data
- 11:08Conclusion
Here we present addressable droplet microarrays (ADMs), a droplet array based method able to determine absolute protein abundance in single cells. We demonstrate the capability of ADMs to characterize the heterogeneity in expression of the tumor suppressor protein p53 in a human cancer cell line.
