Rivista
/
Biologia
/
Kryopræservering af zebrafisk Spermatogonia af hele testiklerne nål nedsænket ultra-hurtig nedkøling
JoVE Journal
Biologia
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

Kryopræservering af zebrafisk Spermatogonia af hele testiklerne nål nedsænket ultra-hurtig nedkøling

DOI:
10.3791/56118-v

•

13:32 min

•

March 04, 2018

•

, , , , ,
1Department of Aquaculture,Szent István University

Capitoli

  • 00:00Titolo
  • 01:14Testis collection
  • 03:29Ultra-rapid cooling of the testicular tissue
  • 06:11Warming procedure
  • 07:56Tissue digestion
  • 10:27Viability evaluation and cell counting
  • 11:28Representative Results
  • 12:20Conclusions

Summary

Traduzione automatica

Traduzione automatica

Det vigtigste formål med denne undersøgelse var at tilpasse nålen nedsænket vitrifikation (NIV) proceduren for at cryopreserve hele zebrafisk testiklerne. Derudover blev repeterbarhed af metoden i fem forskellige zebrafisk stammer testet.

Tags

CryopreservationZebrafishSpermatogoniaVitrificationWhole TestesUltra-rapid CoolingGerm CellsTransplantationSlow-rate FreezingSpermatozoaEuthanasiaDissectionTestes RemovalSterilizationEquilibrationVitrification Solutions

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Transplantation of Whole Kidney Marrow in Adult Zebrafish

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Generating Chimeric Zebrafish Embryos by Transplantation

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

Making Gynogenetic Diploid Zebrafish by Early Pressure

Isolation of Drosophila melanogaster Testes

Kryopræservering af Mouse embryoner ethylenglycol-Based forglasning

Imaging centrosomes i Fly Testiklerne

Analyse af apoptose i zebrafisk embryoner Whole-mount Immunofluorescens Detect Aktiveret Caspase 3

Flat Mount Forberedelse til observation og analyse af Zebrafisk Embryo Enheder Stained af Whole Mount<em> In situ</em> Hybridisering

Read Article