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应用重组蚊子核作为基因载体, 用于蚊幼虫基因功能分析
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
DOI:

12:30 min

October 06, 2017

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Capitoli

  • 00:05Titolo
  • 01:27Designing an Artificial Intronic Small RNA Expression Cassette
  • 04:22Generating a rAaeDV Construct by Cloning a miRNA or shRNA Expression Cassette into a Plasmid Backbone
  • 05:19Transfecting C6/36 Cells with rAaeDV Plasmids
  • 07:33Harvesting rAaeDV Virions from Transfected C6/36 Cells
  • 08:53Mosquito Transduction
  • 10:10Results: Overexpression and Knockdown Efficiency of rAaeDV Vectors
  • 11:14Conclusion

Summary

Traduzione automatica

我们报告使用人工子小 RNA 表达策略, 开发一个无重组的伊蚊蚊核 (AaeDV)体内交付系统。详细介绍了 rAaeDV 载体的构建、包装和定量分析以及幼虫感染的具体步骤。

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