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蚊幼虫における遺伝子の機能解析の遺伝子配達ベクトルとして組換え蚊 Densovirus を使用
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
DOI:

12:30 min

October 06, 2017

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Capitoli

  • 00:05Titolo
  • 01:27Designing an Artificial Intronic Small RNA Expression Cassette
  • 04:22Generating a rAaeDV Construct by Cloning a miRNA or shRNA Expression Cassette into a Plasmid Backbone
  • 05:19Transfecting C6/36 Cells with rAaeDV Plasmids
  • 07:33Harvesting rAaeDV Virions from Transfected C6/36 Cells
  • 08:53Mosquito Transduction
  • 10:10Results: Overexpression and Knockdown Efficiency of rAaeDV Vectors
  • 11:14Conclusion

Summary

Traduzione automatica

良品組換えネッタイシマカdensovirus (AaeDV)生体内での配信システムを開発する、人工イントロン小 RNA 発現戦略を使用して報告します。建設、パッケージング、および幼虫の感染に関しては同様に rAaeDV のベクトルの定量分析のための詳細な手順を説明します。

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