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Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia
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Biologia
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JoVE Journal Biologia
Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia

Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia

DOI:
10.3791/56124-v

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09:23 min

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August 14, 2017

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, , , ,
1School of BioSciences,Bio21 Institute and University of Melbourne

Capitoli

  • 00:05Titolo
  • 00:49General Maintenance and Larval Rearing
  • 03:40Pupal Sexing for Outcrossing
  • 05:00Blood Feeding and Oviposition
  • 07:45Results: The Importance of Good Nutrition to Raising Aedes aegypti Mosquitoes
  • 08:44Conclusion

Summary

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Aedes aegypti mosquitoes infected with Wolbachia are being released into natural populations to suppress the transmission of arboviruses. We describe methods to rear Ae. aegypti with Wolbachia infections in the laboratory for experiments and field release, taking precautions to minimize laboratory adaptation and selection.

Tags

Keywords: Aedes AegyptiWolbachiaMosquito RearingDisease ControlVector ControlPopulation MaintenanceTemperatureHumidityNutritionLarvaePupaeAdult EmergenceSugar SolutionVector Species

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