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Evaluation of Vascular Control Mechanisms Utilizing Video Microscopy of Isolated Resistance Arteries of Rats
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Evaluation of Vascular Control Mechanisms Utilizing Video Microscopy of Isolated Resistance Arteries of Rats

Evaluation of Vascular Control Mechanisms Utilizing Video Microscopy of Isolated Resistance Arteries of Rats

DOI:
10.3791/56133-v

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10:28 min

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December 05, 2017

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, , , , , ,
1Department of Physical Therapy,Marquette University, 2Medical College of Wisconsin, 3Department of Physiology,Medical College of Wisconsin, 4Graduate Programs of Nurse Anesthesia,Texas Wesleyan University, 5Office of Research,Medical College of Wisconsin

Capitoli

  • 00:05Titolo
  • 01:22Solution and Vessel Chamber Preparation
  • 04:47Cannnulated Artery Preparation
  • 08:03Results: Response of Isolated, Cannulated Middle Cerebral Arteries Depends on Both Dietary and Genetic Factors
  • 09:10Conclusion

Summary

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This manuscript describes in vitro video microscopy protocols for evaluating vascular function in rat cerebral resistance arteries. The manuscript also describes techniques for evaluating microvessel density with fluorescently labeled lectin and tissue perfusion using Laser Doppler Flowmetry.

Tags

Vascular Control MechanismsVideo MicroscopyIsolated Resistance ArteriesRatsVascular BiologyEndothelial ControlVascular Smooth Muscle ControlPeripheral Vascular DiseaseCoronary Artery DiseaseMicrocannulationPhysiological Salt SolutionPH AdjustmentCalcium-free SolutionGas Equilibration

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