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Evaluering af vaskulære kontrolmekanismer udnytte Video mikroskopi af isolerede modstand arterierne af rotter
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JoVE Journal Medicina
Evaluation of Vascular Control Mechanisms Utilizing Video Microscopy of Isolated Resistance Arteries of Rats

Evaluering af vaskulære kontrolmekanismer udnytte Video mikroskopi af isolerede modstand arterierne af rotter

DOI:
10.3791/56133-v

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10:28 min

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December 05, 2017

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, , , , , ,
1Department of Physical Therapy,Marquette University, 2Medical College of Wisconsin, 3Department of Physiology,Medical College of Wisconsin, 4Graduate Programs of Nurse Anesthesia,Texas Wesleyan University, 5Office of Research,Medical College of Wisconsin

Capitoli

  • 00:05Titolo
  • 01:22Solution and Vessel Chamber Preparation
  • 04:47Cannnulated Artery Preparation
  • 08:03Results: Response of Isolated, Cannulated Middle Cerebral Arteries Depends on Both Dietary and Genetic Factors
  • 09:10Conclusion

Summary

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Dette manuskript beskriver in vitro- video mikroskopi protokoller til evaluering af vaskulære funktion i rotte cerebral modstand arterier. Håndskriftet beskriver også teknikker til evaluering af microvessel tæthed med fluorescently mærket lektin og væv perfusion ved hjælp af Laser Doppler Flowmetry.

Tags

Vascular Control MechanismsVideo MicroscopyIsolated Resistance ArteriesRatsVascular BiologyEndothelial ControlVascular Smooth Muscle ControlPeripheral Vascular DiseaseCoronary Artery DiseaseMicrocannulationPhysiological Salt SolutionPH AdjustmentCalcium-free SolutionGas Equilibration

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