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1Department of Metabolic Biology,John Innes Centre, Norwich Research Park, 2Department of Botany,University of Wisconsin, Madison, 3Department of Biochemistry and Molecular Biology,Saitama University, 4Precursory Research for Embryonic Science and Technology (PRESTO),Japan Science and Technology Agency (JST), 5Department of Cell and Developmental Biology,John Innes Centre, Norwich Research Park
Capitoli
- 00:05Titolo
- 00:44Arabidopsis Leaf Detachment
- 01:38Fluorescence Microscopy
- 03:06Data Collection
- 05:39Time-course Video Creation
- 06:39Results: Analysis of GCaMP3 Fluorescence to Detect Aphid-elicited Calcium Signals at the Feeding Site in Detached Arabidopsis Leaves
- 07:30Conclusion
Detta protokoll beskriver en enkel metod för att analysera kalcium signaler i växter som genereras av utfodring Ditropis insekter, såsom bladlöss. Arabidopsis thaliana omvandlas med den GFP kalcium biosensor GCaMP3 möjliggör av realtid i vivo imaging av kalcium dynamics med en hög temporal och spatial upplösning.
