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Immunologia e infezioni

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诱导麻痹及视觉系统损伤小鼠 T 细胞特异的视神经脊髓炎抗原水通道蛋白-4

JoVE Journal
Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4

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诱导麻痹及视觉系统损伤小鼠 T 细胞特异的视神经脊髓炎抗原水通道蛋白-4

DOI:

10.3791/56185-v

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09:29 min

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August 21, 2017

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Sharon A. Sagan*^1,2, Andrés Cruz-Herranz*¹, Collin M. Spencer², Peggy P. Ho, Lawrence Steinman, Ari J. Green, Raymond A. Sobel, Scott S. Zamvil²

¹Department of Neurology,University of California, ²Program in Immunology,University of California, ³Department of Neurology and Neurological Sciences,Stanford University, ⁴Department of Pathology,Stanford University

Capitoli

00:05Titolo
01:04Aquaporin 4 (AQP4) Peptide Immunization
03:01T Cell Cutlure and Pro-imflammatory T Cell Polarization
04:35Pathogen AQP4-specific T Cell Adoptive Transfer
05:29In Vivo Optical Tomography (OCT) Retinal Imaging
07:11Results: Representative AQP4 Peptide-specific Th17 Cell Clinical Disease-induction Analyses
09:00Conclusion

Summary

Traduzione automatica

在这里，我们提出一项议定书，引起瘫痪和视神经炎症反应的水通道蛋白 4 (AQP4) 转让-具体从 AQP4^-/-小鼠到 WT 小鼠的 T 细胞。此外，我们演示了如何使用串行光学相干层析成像技术来监视视觉系统功能障碍。

Tags

Keywords: Neuromyelitis Optica Aquaporin-4 T Cells Adoptive Transfer Central Nervous System Autoimmune Disease Optical Coherence Tomography Retinal Injury Complete Freund’s Adjuvant Emulsion Subcutaneous Injection

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