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A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity

A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity

DOI:
10.3791/56191-v

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06:08 min

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August 09, 2017

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, , ,
1UCLA Immunogenetics Center, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine,University of California, Los Angeles

Capitoli

  • 00:05Titolo
  • 00:47Effector Cell and K562 Target Cell Preparation
  • 02:24Effector Cell and K562 Target Cell Co-culture ad Viability Staining and Acquisition
  • 04:23Results: Representative Human NK Cell Cytotoxixity Assessment
  • 05:20Conclusion

Summary

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A flow cytometry-based method to quantitatively determine the cytotoxic activity of human natural killer cells is shown here.

Tags

Keywords: Flow CytometryCytotoxicity AssayNK Cell ActivityNK Cell DeficiencyPBMCK562 CellsCFSEEffector-to-target Cell Ratio

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