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Precise, High-throughput Analysis of Bacterial Growth
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Biologia
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JoVE Journal Biologia
Precise, High-throughput Analysis of Bacterial Growth

Precise, High-throughput Analysis of Bacterial Growth

DOI:
10.3791/56197-v

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09:00 min

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September 19, 2017

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,
1Graduate School of Life and Environmental Sciences,University of Tsukuba, 2Institute of Biology and Information Science,East China Normal University

Capitoli

  • 00:05Titolo
  • 01:02Preparing the Glycerol Stock
  • 03:41Acquiring the Growth Curves
  • 06:35Results: Systematic Analysis of Bacterial Growth
  • 07:24Conclusion

Summary

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Quantitative evaluation of bacterial growth is essential to understanding microbial physiology as a systems-level phenomenon. A protocol for experimental manipulation and an analytical approach are introduced, allowing for precise, high-throughput analysis of bacterial growth, which is a key subject of interest in systems biology.

Tags

Bacterial GrowthHigh-throughput AnalysisSystems BiologyMicrobiologyGrowth CurvesE. ColiEnvironmental BacteriaM63 MediumSpectrophotometryOptical TurbidityGlycerol Stock

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