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丰度和收费水平的转移 Rna 在大肠杆菌中的量化
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli
DOI:

10:34 min

August 22, 2017

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Capitoli

  • 00:05Titolo
  • 00:37Preparation of Spike-in Cells, Harvesting of Experimental Samples and Addition of Spike-in Cells
  • 02:17RNA Preparation
  • 04:44Gel Electrophoresis, Northern Bloting, and Hybridization
  • 07:43Quantifying tRNA and Charging Levels
  • 08:32Results: Quantification of the Abundance and Charging Levels of tRNAs
  • 09:59Conclusion

Summary

Traduzione automatica

在这里,我们提出一种直接测量转移 RNA 从纯化的大肠杆菌RNA 以及方式比较的转运 RNA 相对水平或任何其他短的 RNA,跨越不同的样本基础,增设的穗状花序在收费水平方法单元格引用基因的表达。

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