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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo
 

Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

Article DOI: 10.3791/56218-v • 08:38 min • October 30th, 2017
October 30th, 2017 •
1, 1, 1
1Laboratory of Biochemical Genetics and Metabolism, The Rockefeller University

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Protocols for quantitative assessment of lymphocyte chemotaxis and migration are important tools for immunology research. Here, an in vitro protocol is described that permits real-time, multiplexed evaluation of cell migration, as well as a complementary in vivo technique enabling tracking of native cells to spleen.

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Keywords: Monocyte Chemotaxis In Vitro In Vivo Lymphocyte Trafficking Immunology THP-1 Cells Fluorescent Labeling Cell Migration MCP-1 HEPES RPMI-1640 C57BL/6 Mouse Adoptive Transfer
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