Rivista
/
Biologia dello sviluppo
/
斑马鱼胚胎卵黄细胞中的 AFM 和 Microrheology
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
AFM and Microrheology in the Zebrafish Embryo Yolk Cell
Please note that all translations are automatically generated. Click here for the English version.

斑马鱼胚胎卵黄细胞中的 AFM 和 Microrheology

DOI:
10.3791/56224-v

•

09:47 min

•

November 29, 2017

•

, , , , ,
1Instituto de Biología Molecular de Barcelona,Consejo Superior de Investigaciones Científicas, 2Institute for Bioengineering of Catalonia,Universitat de Barcelona and CIBER Enfermedades Respiratorias

Capitoli

  • 00:05Titolo
  • 01:08Atomic Force Microscopy
  • 03:54Particle Tracking Microrheology
  • 07:02Results: Cortical Tension Measurements and Rheology of the Cortex and Yolk of the Zebrafish Embryo Yolk Cell During Epiboly
  • 08:46Conclusion

Summary

Traduzione automatica

Traduzione automatica

缺少用于测量材料属性和拉伸参数的工具在体内可防止在开发过程中验证它们的角色。我们使用原子力显微镜 (AFM) 和纳米粒子跟踪来量化外包期间完整的斑马鱼胚胎卵黄细胞的机械特性。这些方法是可靠的, 广泛适用于避免介入干预。

Tags

Keywords: AFMMicrorheologyZebrafish EmbryoYolk CellMechanical PropertiesStiffnessViscosityAdhesionMechanobiologyEmbryo MountingEmbryo HandlingAgaroseLow Melting AgaroseInverted Optical MicroscopeAtomic Force Microscope

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/56224?article_type=v

微珠植入斑马鱼胚胎
check_url/it/56224?article_type=v

斑马鱼幼虫骨骼肌诚信与伊文思蓝分析
check_url/it/56224?article_type=v

在活斑马鱼胚胎成像的亚细胞结构
check_url/it/56224?article_type=v

在GFP-转基因斑马鱼使用Photoconvertible PSmOrange系统追踪单元
check_url/it/56224?article_type=v

日本鹌鹑胚胎的卵黄囊膜主内胚上皮细胞培养
check_url/it/56224?article_type=v

观察有丝分裂和动力学在现场斑马鱼胚胎
check_url/it/56224?article_type=v

通过流式细胞术鉴定小鼠胚胎中的红细胞生成素及其后代
check_url/it/56224?article_type=v

利用 Immunolabeling 分析斑马鱼胚胎中的稳定、动态和初生的微管
check_url/it/56224?article_type=v

在斑马鱼胚胎中通过细胞 Photoconversion 的心内膜组织运动后
check_url/it/56224?article_type=v

家兔模型最佳胚胎阶段微创胚胎移植与胚胎玻璃化

Read Article