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利用自动 Microbioreactor 技术优化异种蛋白质生产的通用协议
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
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利用自动 Microbioreactor 技术优化异种蛋白质生产的通用协议

DOI:
10.3791/56234-v

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06:24 min

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December 15, 2017

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, , , ,
1IBG-1: Biotechnology,Forschungszentrum Jülich, 2Research Center Jülich,Bioeconomy Science Center (BioSC), 3Computational Systems Biotechnology (AVT.CSB),RWTH Aachen University, 4Institute for Biotechnology,RWTH Aachen University

Capitoli

  • 00:05Titolo
  • 01:05Seed Culture, Automated Media Preparation, and Start of Main Culture
  • 02:53Product Quantification and Preprocessing of Raw Data for Analysis
  • 04:22Results: Iterative Workflow with Final Statistical Evaluation of Results
  • 05:31Conclusion

Summary

Traduzione automatica

Traduzione automatica

这份手稿描述了 tailor-made 设计微生物培养介质的通用方法。这是由一个迭代工作流, 结合 Kriging-based 实验设计和 microbioreactor 技术的充分栽培吞吐量, 这是支持的实验室机器人, 以提高可靠性和速度在液体处理介质制备.

Tags

Heterologous Protein ProductionAutomated Microbioreactor TechnologyKringing Based Experimental DesignMicrobial Cultivation Media OptimizationBioprocess DevelopmentStrain PhenotypingDeep Well PlatesSeed CultureRobotic WorkflowMedia PreparationOptical DensityMain CulturesCultivation Microtiter PlatesBiolector DeviceStock Solutions DisposalLabware CleaningLiquid Handler Decontamination

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