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Etablering af larve zebrafisk som en dyr Model til at undersøge Trypanosoma cruzi motilitet In Vivo
JoVE Journal
Biologia dello sviluppo
Author Produced
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo
DOI:

13:21 min

September 30, 2017

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Capitoli

  • 00:00Titolo
  • 00:13Introduction
  • 03:09Obtaining Embryos for Injection
  • 04:13Preparing Injection Material
  • 05:38Obtaining Labeled T. cruzi Parasites for Injection
  • 07:04Injecting Zebrafish Larvae
  • 08:10LSFM Mounting and Imaging of Injected Larvae
  • 08:57LSFM Imaging of Injected Larvae
  • 10:44Representative Results
  • 11:40Conclusion

Summary

Traduzione automatica

I denne protokol, fluorescently mærket T. cruzi blev sprøjtet ind i gennemsigtige zebrafisk larver og parasit motilitet blev observeret i vivo ved hjælp af lys ark Fluorescens mikroskopi.

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