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Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish
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Neuroscienze
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JoVE Journal Neuroscienze
Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish

Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish

DOI:
10.3791/56249-v

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06:27 min

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October 27, 2017

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, , , ,
1Department of Ophthalmology, University Hospital of Bern,University of Bern, 2Department of Clinical Research,University of Bern, 3Graduate School for Cellular and Biomedical Sciences,University of Bern

Capitoli

  • 00:05Titolo
  • 00:48Laser Focal Injury on Zebrafish Retina
  • 02:31In Vivo Imaging of Retinal Morphology
  • 03:59Results: Real-time Optical Coherence Tomography
  • 05:43Conclusion

Summary

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The zebrafish is a popular animal model to study mechanisms of retinal degeneration/regeneration in vertebrates. This protocol describes a method to induce localized injury disrupting the outer retina with minimal damage to the inner retina. Subsequently, we monitor in vivo the retinal morphology and the Müller glia response throughout retinal regeneration.

Tags

Müller GliaRetinal DegenerationRetinal RegenerationLaser-induced InjuryZebrafishIn Vivo ImagingOptical Coherence Tomography (OCT)AnesthesiaTricaineHydroxypropyl MethylcelluloseFundus LaserHydrogel Contact Lens

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