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糖注射水凝胶的制备及其在3D 细胞培养中的应用

JoVE Journal
Chimica

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JoVE Journal Chimica

Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture

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糖注射水凝胶的制备及其在3D 细胞培养中的应用

DOI:

10.3791/56253-v

•

08:05 min

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September 29, 2017

•

Yongsan Li, Yaling Zhang, Yen Wei, Lei Tao

¹The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry,Tsinghua University, ²Institute of Chemical Materials,China Academy of Engineering Physics

Capitoli

00:05Titolo
00:59Synthesis of DF PEG for Hydrogels
03:00Preparing Chitosan-based Hydrogels with Encapsulated Cells
05:15Cell Viability Analysis
06:08Results: Affect of Injection on Viability of Hydrogel-encapsulated L929 Mouse Fibroblasts
07:29Conclusion

Summary

Traduzione automatica

在这里, 我们描述了一个轻便的制备糖注射水凝胶使用动态胺化学。介绍了水凝胶的机械强度调整方法及其在3D 细胞培养中的应用。

Tags

Chitosan Injectable Hydrogel 3D Cell Culture Self-healing Hydrogel DF PEG Benzaldehyde-terminated DF PEG Cell Encapsulation Cell Culture Media Cell Therapy Carrier

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