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CRISPR/Cas9 复合原注射液生产基因工程金黄叙利亚仓鼠
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex
DOI:

06:12 min

January 09, 2018

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Capitoli

  • 00:05Titolo
  • 01:06Zygote Isolation
  • 03:31PN Injection
  • 04:52Results: Evaluation of Gene Targeting in Golden Syrian Hamster by the Cas9/sgRNA System
  • 05:36Conclusion

Summary

Traduzione automatica

原 (PN) 注射的聚类定期 interspaced 短复发重复 (CRISPR) 和 CRISPR 相关 protein-9 核酸 (CRISPR/Cas9) 系统是一种高效的方法生产基因工程金黄叙利亚仓鼠。在此, 我们描述了详细的 PN 注射协议生产的基因剔除仓鼠与 CRISPR/Cas9 系统。

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