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人胚胎或诱导多能干细胞对视网膜色素上皮细胞的快速定向分化
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
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人胚胎或诱导多能干细胞对视网膜色素上皮细胞的快速定向分化

DOI:
10.3791/56274-v

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10:48 min

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October 30, 2017

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,
1Center for Stem Cell Biology and Engineering,University of California, Santa Barbara

Capitoli

  • 00:05Titolo
  • 01:02Day 0: Day of Pluripotent Stem Cell Passage for Differentiation
  • 04:43Day of Enrichment to Passage 0 of Retinal Piment Epithelial Cells
  • 08:37Results: Representative Morphology and Confluence of Maturing RPE
  • 09:48Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议描述了如何产生多能干细胞的视网膜色素上皮细胞 (RPE)。该方法结合生长因子和小分子, 将干细胞分化为未成熟的 rpe, 在十四天后成熟, 功能性视网膜色素上皮细胞三月后。

Tags

Retinal Pigment Epithelial CellsHuman Embryonic Stem CellsInduced Pluripotent Stem CellsRetinal DifferentiationDirected DifferentiationStem Cell BiologyEye ResearchRPERetinal DevelopmentStem Cell Disease ModelGrowth FactorsSmall MoleculesExtracellular MatrixHydrogelEDTACell PassagingCell RemovalUndifferentiated Stem CellsEfficiencyEnrichment

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