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Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy

Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy

DOI:
10.3791/56284-v

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09:24 min

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November 29, 2017

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, , , ,
1EPSRC Proteus Hub, MRC Centre of Inflammation Research, Queen’s Medical Research Institute,University of Edinburgh, 2School of Chemistry, EaStChem,University of Edinburgh

Capitoli

  • 00:05Titolo
  • 00:44Preparation of Ex Vivo Human Lung Tissue
  • 01:53Setup of the Confocal Laser Endomicroscopy (CLE) Device
  • 03:55Data Collection with CLE
  • 05:59Data Analysis
  • 07:41Results: FCFM Imaging of Labeling Bacteria with Human Lung Tissue
  • 08:28Conclusion

Summary

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This technique describes an efficient screening process for evaluating bacteria-specific optical imaging agents within ex vivo human lung tissue, by fibered confocal fluorescence microscopy for the rapid identification of small molecule chemical probe-candidates with translatable potential.

Tags

Optical ImagingConfocal Laser EndomicroscopyBacteria-specific ProbesMolecular ImagingImaging ProbesCalcein AM-labeled BacteriaFibered Confocal Fluorescence MicroscopyLaser Scanning UnitFCFM Imaging Fiber Calibration

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