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Journal / Neuroscience / Live Imaging Followed by Single Cell Tracking to…
JoVE Journal
Neuroscience

This Contenuto is Open Access.

JoVE Journal Neuroscience
Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations
 

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Article DOI: 10.3791/56291-v • 10:55 min • December 16th, 2017
December 16th, 2017 •
*1,2,3, *1,2,3, *4,5, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 6, 7, 1,2,3, 1,2,3
1Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University, 2University Institute for Neurochemistry Research (IUIN), 3Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 4Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center, Ludwig-Maximilians University Munich, 5Toxicology and Pharmacology Department, Faculty of Veterinary medicine, Complutense University, 6Brain Institute, Federal University of Rio Grande do Norte, 7Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

A robust protocol to monitor neural populations by time-lapse video-microscopy followed by software-based post-processing is described. This method represents a powerful tool to identify biological events in a selected population during live imaging experiments.

Tags

Live Imaging Single Cell Tracking Cell Biology Lineage Progression Neural Populations Post-imaging Immunocytochemistry Neuroscience Cell Behavior Modulators Variations In Cell Biology End Point Analysis Culture Condition Acquisition Software Plating Immunocytochemistry Mechanisms Critical Biological Events Proliferation Differentiation Cell Fate Decisions Therapeutic Strategies Nervous System
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