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Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
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JoVE Journal Biologia dello sviluppo
Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

DOI:
10.3791/56308-v

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07:35 min

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January 24, 2018

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, , , ,
1Department of Cell Biology,University of Oklahoma Health Sciences Center, 2Department of Ophthalmology/Dean McGee Eye Institute,University of Oklahoma Health Sciences Center, 3Department of Physiology, Harold Hamm Diabetes Center,University of Oklahoma Health Sciences Center

Capitoli

  • 00:05Titolo
  • 01:17Isolation of Primary Cells
  • 04:04Primary HCF Culture and 3D Construct Assembly
  • 05:00Cell Differentiation and Co-culture
  • 06:17Results: Transmission Electron Microscopy of the 3D Self-assembled Construct
  • 06:48Conclusion

Summary

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This protocol describes a novel three-dimensional in vitro model, where corneal stromal cells and differentiated neuronal cells are cultured together to assist in the examination and understanding of the interactions of the two cell types.

Tags

Stromal-nerve InteractionsHuman CorneaCorneal DiseasesCorneal DystrophiesExtracellular MatrixCell-cell InteractionsDrug ScreeningCorneal Nerve DamageCorneal Nerve Regeneration

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